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Analysis of Acanthamoeba genotypes from public freshwater sources in Thailand reveals a new genotype, T23 Acanthamoeba bangkokensis sp. nov.

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Overview

Paper Summary › Explain Like I'm Five › Conflicts of Interest › Identified Limitations › Rating Explanation › Good to know › Topic Hierarchy › File Information ›

Paper Summary

Paperzilla title
New amoeba species found swimming in Bangkok!

A survey of public freshwater sources in Thailand identified a new genotype of Acanthamoeba, named T23 Acanthamoeba bangkokensis sp. nov. Genotype T4 was the most common, and evidence suggested purifying selection and intragenic recombination play a role in the evolution of the 18S rRNA gene in this genus.

Explain Like I'm Five

Scientists found tiny, invisible living things called Acanthamoeba in Thailand's water. They discovered a brand new type, like finding a new animal species, and named it!

Possible Conflicts of Interest

None identified

Identified Limitations

Limited geographical scope within regions
The limited sampling within each region (3-7 provinces) may not fully represent the true genotypic diversity of Acanthamoeba in those regions, potentially leading to an underestimation of the actual variation.
Sole reliance on 18S rRNA gene
Relying solely on 18S rRNA gene sequencing for genotype identification might overlook other genetic markers that could contribute to a more comprehensive understanding of Acanthamoeba diversity and pathogenicity.
Limited investigation of cryptic subtypes
The study acknowledges the possibility of cryptic subtypes within genotypes based on nucleotide diversity but doesn't fully investigate or characterize these potential subtypes, leaving a gap in understanding the true diversity.
Limited exploration of recombination mechanisms
While the study found evidence of intragenic recombination, the exact mechanisms and implications of this recombination on the evolution and diversity of Acanthamoeba are not fully explored.

Rating Explanation

This study provides valuable insights into the genotypic diversity and distribution of Acanthamoeba in Thailand's freshwater sources, including the discovery of a new genotype. The methodology is robust, employing both morphological and molecular characterization. The study's limitations, such as the limited geographical scope within regions and the sole reliance on 18S rRNA gene for genotyping, are acknowledged. Overall, the research significantly contributes to the understanding of Acanthamoeba diversity and evolution, warranting a rating of 4.

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File Information

Original Title: Analysis of Acanthamoeba genotypes from public freshwater sources in Thailand reveals a new genotype, T23 Acanthamoeba bangkokensis sp. nov.
Uploaded: July 14, 2025 at 11:20 AM
Privacy: Public