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LATS kinase-mediated CTCF phosphorylation and selective loss of genomic binding

★ ★ ★ ★ ☆

Paper Summary

Paperzilla title
Stressed Cells Rearrange Their DNA: Turning Off Growth Genes

This study found that under cellular stress, a specific enzyme (LATS kinase) modifies a DNA-organizing protein (CTCF), causing it to detach from certain growth-promoting genes. This detachment alters the 3D structure of DNA, leading to the downregulation of those genes. The research was primarily conducted in cell lines.

Explain Like I'm Five

Stressed cells change their DNA organization to turn off growth genes. It's like rearranging furniture in a room to make it less inviting.

Possible Conflicts of Interest

None identified

Identified Limitations

Limited in vivo validation
The study primarily relies on in vitro experiments and cell line models, which may not fully reflect the complexity of in vivo systems and the diverse range of cellular responses to stress.
Correlation does not equal causation
While the study demonstrates a correlation between CTCF phosphorylation, loss of DNA binding, and downregulation of YAP target genes, further investigation is needed to fully establish a direct causal relationship.

Rating Explanation

This study provides compelling evidence for a novel mechanism by which cellular stress can dynamically modulate 3D genome architecture and gene expression through LATS-mediated CTCF phosphorylation. The research employs a robust combination of biochemical, cell biological, and genomic approaches, significantly advancing our understanding of the interplay between cellular signaling, chromatin topology, and gene regulation. While further in vivo validation is needed to strengthen the findings, the study's strengths in methodology and the novelty of the discoveries warrant a rating of 4.

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File Information

Original Title: LATS kinase-mediated CTCF phosphorylation and selective loss of genomic binding
Uploaded: August 04, 2025 at 01:44 PM
Privacy: Public