Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane-Membrane Contact Sites

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Conflicts of Interest

Identified Weaknesses

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Paper Summary

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Glowing Proteins Light Up Calcium Hotspots in Cells

This study developed new self-complementing bipartite fluorescent proteins and calcium biosensors that can be used to visualize calcium dynamics at membrane-membrane contact sites in living cells. These biosensors demonstrated successful monitoring of calcium transfer between the endoplasmic reticulum and mitochondria, highlighting their potential for studying organelle communication. Further in vivo validation and investigation of long-term effects are needed.

Possible Conflicts of Interest

None identified.

Identified Weaknesses

Limited in vivo validation

The study primarily relies on in vitro and cell culture experiments, which may not fully represent the complexity of in vivo systems.

Limited scope of signaling pathways

The study focuses solely on calcium signaling and does not explore the interplay with other signaling pathways.

Lack of long-term stability and toxicity assessment

Although the biosensors show promising results, their long-term stability and potential toxicity in vivo need further investigation.

Rating Explanation

The paper presents a novel approach to developing self-complementing bipartite fluorescent proteins and calcium biosensors with good performance. The study provides valuable tools for visualizing and studying calcium dynamics at membrane-membrane contact sites, offering potential for broader applications in cell biology research. However, further investigation is needed to validate the in vivo applicability and long-term stability of the biosensors.

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