Limited clinical relevance of visual function improvements
The study heavily relies on visual function improvement as a primary outcome measure. While there are statistically significant changes observed in color contrast sensitivity, the clinical significance of these improvements is unclear. A 9% or 16% improvement in color contrast thresholds may not translate to a noticeable or meaningful difference in daily visual function for the participants.
The sample sizes for the different experiments are relatively small (N=7 for sunlight, N=13 for 850 nm light, N=5 for body-only exposure, and N=7 for control). Small sample sizes increase the risk of type II errors (false negatives) and limit the generalizability of the findings to larger populations.
Limited understanding of long-term effects and optimal dosage
The study uses a single, 15-minute exposure to 850 nm light. The long-term effects of this wavelength, as well as the optimal dosage and duration of exposure for maximizing visual benefits, remain unknown.
Limited understanding of underlying mechanisms
While the study demonstrates that 850 nm light can penetrate the body and potentially improve visual function, the underlying mechanisms responsible for this effect are not fully elucidated. The authors suggest a link to improved mitochondrial function, but more research is needed to confirm this hypothesis and explore the specific pathways involved.
Limited evaluation of safety and potential risks
The study does not adequately address the potential risks and side effects associated with exposure to 850 nm light. While infrared light is generally considered safe, more research is needed to determine the potential long-term effects of repeated or prolonged exposure.