The study acknowledges that additional research is needed to confirm some observations, such as the higher Sertoli cell to spermatogonia ratio in rescued males, through quantitative morphological analyses. It also recognizes the need to determine the functionality of mutated dnd sequences in developing sperm or eggs in salmon. Furthermore, the study plans to cross rescued dnd crispants to obtain homozygous mutants for the most common dnd loss-of-function variant, which is crucial for confirming homozygous mutations in the whole gonad.

The study is limited to a relatively small sample size, particularly in the histological analyses and deep sequencing portions. Larger sample sizes would be beneficial to confirm the observed trends and mutation patterns with greater statistical power.

While the study demonstrates germ cell rescue in one-year-old salmon, it does not investigate long-term effects or functionality beyond this stage. Additional studies on the maturation of rescued germ cells and their ability to produce viable offspring are necessary to confirm the long-term success of this method.