This study systematically documented morphological alterations in zebrafish embryos after exposure to 48 chemicals, revealing a range of common and specific developmental defects. The findings emphasize the importance of considering a wide spectrum of morphological endpoints beyond those listed in the OECD TG 236 guidelines for a more comprehensive assessment of chemical toxicity in zebrafish embryos.

The authors received funding from the European Union's Horizon 2020 research and innovation program, but no specific conflicts of interest were declared.

This study provides a valuable resource for zebrafish embryotoxicity testing by cataloging a wide range of morphological effects observed after exposure to various chemicals. It highlights the need for a standardized nomenclature and emphasizes the importance of considering sublethal endpoints for a comprehensive toxicity assessment. Although the study focuses on morphological observations and does not delve into underlying mechanisms, it lays the groundwork for future mechanistic studies. The study's methodology is sound, and the results are clearly presented. There is a slight concern regarding potential bias due to funding source but appears to be negligible.