Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon
This study rescued germ cells in genetically modified sterile Atlantic salmon by co-injecting wild-type dnd mRNA with CRISPR-Cas9 constructs targeting the dnd gene. Rescued one-year-old fish contained germ cells in their gonads, despite high dnd mutation rates, suggesting a potential pathway for large-scale production of sterile salmon offspring through genetically sterile broodstock.